Effects of Different Pulsing Characteristics on Transformation Efficiency Via Electroporation
نویسنده
چکیده
This study will focus on how the different characteristics of the applied pulse, including voltage, pulse length, frequency and multiple pulsing affect transformation efficiency of Top10 E-coli cells. It will be shown that transformation efficiency will be maximal at optimum settings different from that suggested by electroporator manufacturer. Experimental results will also verify pore formation model developed by Schoenbach et al.
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